GFP anticorps

N° du produit ABIN350304

Cet anticorps anti-GFP est un anticorps Lapin Polyclonal détectant GFP dans WB et IHC. Adapté pour Aequorea victoria.

Aperçu rapide pour GFP anticorps (ABIN350304)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GFP anticorps

Fonction: Rabbit antibody to EGFP/GFP/Venus

Specificité: Specific for EGFP/GFP/Venus

Réactivité croisée (Details): Any organism expressing EGFP/GFP/Venus.

Purification: IgG

Immunogène: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. A working concentration of 5-50 μg,ml is recommended. The optimal concentration should be determined by the end user.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term.

Date de péremption: 12 months

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Sujet: GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of  aequorin's blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.